A multi-capillary inlet to focus ions and other charged particles generated at or near atmospheric pressure into a relatively low pressure region, which allows increased conductance of ions and other charged particles. The aft and forward gripper assemblies are adapted for selective engagement with the inner surface of the borehole. The master disks can be aligned while the alignment marks are externally observed. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. The method includes a step of diluting a concentrate located in the metering apparatus by taking up a diluting agent from the extracorporeal circuit.