A method and apparatus for electrically interfacing between two or more distinct environments. The IC package, the PCB and the motherboard are subjected to thermal, humidity andor electrical test conditions. The nucleic acids encoding specific siRNAs and vectors encoding these siRNAs are useful as tools to specifically inhibit the expression of Pit-1, Pit-2, or related proteins in various primary and stably-transformed cell lines, and as tools to explore the roles and functional relationships of these proteins involved in vascular calcification.