According to a method for transferring a functional region, at least part of functional regions on separation layers arranged on a first substrate is transferred onto a second substrate, the separation layers being capable of being brought into a separable state by treatment. Calcified plaque segmentation can be used to estimate the distribution of superficial calcification and inform strategies stenting. When the containers are closed and pressure is applied to the outer container, ambient fluid trapped within the outer container transfers the pressure to the inner container, thereby urging fluid flow from the inner container, through the one-way valve, and out of the packaging device. % of a filler, such as, aluminum hydroxide, 0 to 40 wt. The invention further relates to hybrid soybean seeds, plants and plant parts produced by crossing the cultivar 82224905 with another soybean cultivar.