A method of reducing the content of TSNA in tobacco leaves, comprising treating the tobacco leaves with a microorganism having no nitrate-reducing ability but having the ability of growth-competition with a microorganism belonging to Enterobacter or Pantoea genus. The method according to the invention comprises the following steps: a) the sample is held in an aqueous solution; b) lysing of the sample; c) separation of cellular debris; and d) the nucleic acids are isolated from the solution, steps to taking place under non-chaotropic conditions. The foldable actuator can be foldable between an insertion configuration for placement through an access device without the aid of a grasper or applicator, and a deployed configuration for supporting the tissue retrieval bag in an open state. A preferred approach includes isolating the motherboard from its mounting harnesses and adding an echo damping and shock absorption pad to the underside of the enclosure cover to stiffen the enclosure cover while maintaining its light weight.