The resonator comprises a piezoelectric layer arranged between two electrodes. The methods comprise flowing a sample with a series of particles through the flow cytometer, detecting events and calculating an expected frequency of those events based on a distribution, such as a Poisson distribution, and measuring an observed frequency of particle events. The sensing unit senses a variation in temperature to generate a temperature difference signal, while the control unit executes a program code to determine a non-trigger range based on the ambient temperature. The increased database accuracy subsequently improves the quality of position location measurements from the wireless network. An outer surface of each of the pivot shafts engages with an inner surface of one of the elastic sleeves. The content manager causes the bucket to receive the content object and presents representations of the buckets to a user.