An imaging apparatus includes an imaging unit having a first mode for generating a first video signal according to a first video signal format, and a second mode for generating a second video signal according to a second video signal format. The system contains a first tier queue associated with a server based mechanism for directing service requests to a second tier queue associated with each of the one or more query servers. The present invention is based on a special calibration procedure for determination of a channel cross-talk matrix and enables an accurate separation of the fluorescence emitted from individual capillary lanes.