A connector means for tie-in and connecting of a first pipeline and a second pipeline on the seabed is shown. In one embodiment, the applicator comprises tube having an interior and a flexible material which has one end that is formed into a flexible and expandable loop. The apparatus controls a rotation speed of the compressor such that the volume flow rate in the heating operation is greater than or equal to the heating-mode lower limit refrigerant volume flow rate. The shape memory alloy actuator is heat activated to distort the shape memory actuator from a first shape to a second shape to activate the force applying member to act upon the force receiving member to move the force receiving member to a different position relative to the force applying member. One computer-implemented method includes initiating a start of a user interface following receipt of a theming request, requesting available themes for presentation in the user interface, receiving a request to access an existing theme, receiving theming data responsive to a generated request to load the existing theme, and receiving a request to publish modified theming data. The resulting slide is useful in the preparation of highly homogeneous functional-group slides and the high-density and high-efficiency bio-chipmicroarray.