One preferred embodiment includes a guidewire manipulation device for manipulating a guidewire during a procedure. Also provided are methods for treating cancer provided herein, comprising administering to a mammal diagnosed with cancer, substantially non-viable Gram-negative bacteria having a substantial reduction in endotoxin activity andor pyrogenicity, in an amount sufficient to inhibit growth or metastasis of the cancer. The system determines a transit time for a signal interconnecting the functional blocks. Parameters which define the relationship are supplied to the controller from a remote data processing system which analyses flow rate and output pressure data transmitted from the controller, and also pressure data from a remote sensor at the critical point.