A device positions an ultrasound transducer for ultrasound therapy to focus a treatment beam emitted by the ultrasound transducer at tissue of interest. A first type of request is received from a disk-based file system to access a first type of storage device. Intensity of a plurality of pixels of a plurality of scan lines of a field of the selected frames is altered according to at least one pattern or as designated by an adjustment. The disposition indicator is then captured in conjunction with other instruction trace information for subsequent post-processing. The invention is also methods of purifying protein-containing-polymeric-microspheres from unused polymer, and an apparatus therefore. The inventive marking system is used to identify and authenticate articles, products or objects.