An information network, such as an interactive set-top box, is provided to a subscriber. A server computing device receives a request for media content from a remote computing device. A population of mutant proteins can be created in which an amino acid residue has replaced an existing residue at random locations along the primary sequence of the protein. Test signals are transmitted by the resource server and, after being mirrored at one or two gateways, are transmitted back to the resource server. From the resist ridge that has been formed as a line in the photosensitive resist, in a type of tone reversal, an opening has been formed in the hard mask layer and a trench has been formed in the layerlayer stack.