The present invention is directed to immobilized biologically active entities that retain significant biological activity following sterilization of the immobilized biologically active entities. The invention also includes an improved electrostatic apparatus for dewatering andor desalting a water-in-oil emulsion comprising a plurality of horizontally directed nozzles positioned to introduce a plurality of streams of the emulsion into the electrostatic field under opposed flow conditions. The envelope may include a drug coating, positioning aids, and means to ease envelope removal. A next step 508 includes determining whether a maximum for the at least one operating parameter exceeds 510 the upper bound. Inbred maize lines derived from inbred maize line PH8JR, methods for producing other inbred maize lines derived from inbred maize line PH8JR and the inbred maize lines and their parts derived by the use of those methods.