The present invention comprises methods and compositions for the reduction of oxalate in humans, and methods for the purification and isolation of recombinant oxalate reducing enzyme proteins. The vacuum pipe conveys the material to be collected into a collecting container connected to the vacuum pipe. The color distortion data includes hues of the distorted color identifying which color affected such a distortion and intensity data representing how much color distortion data affected such a distortion. The numerous MR images for the numerous time segments are reconstructed from the acquired raw data, wherein the reconstruction occurs in an iterative process and with a temporal regularization, which is dependent on the mask. 0; optimal temperature: 55\xb0 C.