In a DBA method for an E-PON including an OLT and ONUs, DBACNs corresponding to processes in a tree algorithm are arranged in multi-level stages with the ONUs being in the lowermost stage. Antigen may induce a specific immune response in the subject. For instance, reversibly protected silanes are of particular value in applications where room temperature cure andor adhesion is of value, such as coatings, high resolution imaging, caulks, adhesives, sealents, gaskets, and silicones.