A light emitting diode having high light extraction efficiency and a method of manufacturing the same are provided. A destination is retrieved in response to selection of the destination by the user. Sequential electrical power delivery from the generators is achieved via a sequencing means. ; G1 and G2 are independently a divalent C1-C20 aliphatic group etc. The methods, combinations, and collections are useful for determining whether a vaccine is effective and, for example, for comparing the efficacy of different vaccines.