A method of preparing a toner includes: preparing a toner composition including a macromonomer having a hydrophilic group, a hydrophobic group and at least one reactive functional group, at least one polymerizable monomer, and a colorant; emulsion-polymerizing the toner composition in a medium; and separating and drying the polymerized toner. The lock body includes at least one positioning post mounted thereon for fitting with at least one pull piece by using at least one through hole of the at least one pull piece. This coupling allows more precise rotational control of the assembly when it is manipulated at its proximal end. Component POI scores for each of the component POIs are calculated as a function of a distance between the locations of the component POI and the location of the target POI. These approaches typically affect the level of expression, the ease of purification, the cellular localisation, andor the immunological properties of the expressed protein.