Methods of engine system control responsive to oxygen concentration estimated from engine cylinder pressure. The cultivation substructure is isolated as a subchamber of its own that is separate from the culture chamber, and imaging is carried out by moving the imaging optics and the subchamber in relation to each other. When the split control pad is bonded with outside Vdd or Vss, both sections output \u201c1,1\u201d or \u201c0,0\u201d respectively. Then, all of the entries are stored in the corresponding allocated storage locations according to prefix length.