A machine and method for performing balancing measurements on a tire or tirewheel assembly that includes a structure defining spaced apart tire centering and tire testing positions. The target module is prepared by binding selectively a target protein, modified to allow its detection, to a surrogate ligand that is linked to an individually detectable bead. In addition, a method for identifying IGF-1 indirect agonists is provided using a detergent as a standard for the level of inhibition of binding of IGFBP-1 or IGFBP-3 to IGF-1 andor using the coordinates of the binding pockets of IGF-1 to which a candidate indirect agonist binds for structure-based drug design. The flux collected to a center portion of the motor base 16 by the rotation drive of the fan is flown to the drain portion 20 formed in the center portion of the motor base 16, is flown along the inclined surface 20A and contained into a collection container 34 from the drain portion 20 through the discharge port 46, a drain pipe and a pipe 48. The method offers significantly increased pattern recognition capability, which provides greater automation potential and reduced missed detection and false alarm rates.