The present disclosure relates to a tissue repair assembly. The present invention also provides a novel method for the synthesis of alpha-amino acids and derivatives thereof. A trench is formed from the surface of the semiconductor diffusion layer to a depth reaching the second semiconductor layer. The fusion-bonded area is formed in an entire circumference in gastight effect at an area outside an outer periphery of the ventilation bore and has no clearance, wherein suppose that the ventilation bore has an opening surface area A at the end face of the seal member and a region surrounded with the fusion-bonded area has a surface area B, the relationship is established as A<B\u22669A. The system also provides proprietary access to the database by qualified users through an internet portal. In another embodiment, a continuous measurement can be made using two wavelengths that are both sensitive to oxygen saturation, but they both are equally sensitive.