A method of single parameter and multiparameter characterizing of cells, particularly immunophenotyping of cells, is provided. More particularly, the invention relates to a four-sided, solid pitching rubber which provides an all-in-one durable construction with improved performance characteristics. The decoder receives undecoded I and Q symbols from the plurality of encoded data signals and outputs Euclidian distances corresponding to each encoded signal. This pressure increase can be used to expel gas from the container via the piston outlet and to distribute liquid from the container via the piston outlet andor another outlet.