This invention relates, in part, to unique and newly identified genetic polynucleotides involved in the process of bone remodeling, variants and derivatives of the polynucleotides and corresponding polypeptides, uses of the polynucleotides, polypeptides, variants and derivatives, and methods and compositions for the amelioration of symptoms caused by bone remodeling disorders. In the method a soft grip member is provided having an elastomeric wall affixed to a rigid wall. Another aspect of the invention provides a method of creating a separation cartridge having varying hydrophobicity. In addition, the systems and methods provide secure file transfers between an agent and a transaction card issuerprocessor.