An anastomosis device is provided for making connections between various conduits in the body of a patient. More precisely, the present invention relates to a PCR product cloning vector designed to be effective in use for bluewhite colony selection and produced based on the restriction enzyme treatment process to generate non- complementary unpaired single overhang and a method for producing the same. For example, an amplifier with crossover distortion will show increased harmonic, cross modulation, triple beat, and or intermodulation distortion near the zero crossing while providing less distortion in other portions of the transfer curve of the amplifier.