Methods and compositions for inducing mitochondrial biogenesis are provided. The gel comprises water, thickening agent, and biodiesel by-product glycerin comprising glycerol and soap. An alternative, single piece shroud is also provided which can be retrofitted to an existing cooling system. In an alternative embodiment an isolator valve sleeve is utilized for instances wherein the isolator valve bore has extreme wear that cannot be corrected solely by the installation of the replacement isolator valve.