A closure assembly includes a valve for containing a substance in a squeeze bottle, a multi-layer material for providing a valve for such a closure assembly, a method for providing such a multi-layer material and a method for providing such a closure assembly. Optionally, an undercoat layer is printed on the substrate prior to applying the first ink jet ink. When in the substantially upright position, the flanges are securely connected to define a baking zone with base plate and the flanges. Example secondary factors include a time of day of the input; andor a machine identification, location, and IP address of the computer used to input the sample. The present invention also relates to purified preparations of embryonic stem cells and for uses of embryonic stem cells in treating a wide variety of conditions, diseases and disorders.