A eukaryotic expression vector capable of displaying a multi-chain polypeptide on the surface of a host cell is provided, such that the biological activity of the multi-chain polypeptide is exhibited at the surface of the host cell. The invention reduces processing overhead within a mainframe computing environment were individual requests are treated as individual transactions by instead storing requests within a queue, spawning necessary processes according to the queue and processing multiple transactions in a pseudo batch manner. The knitted loops of the first wale are supplied to a front needle bed, and the knitted loops of the second wale are supplied to a back needle bed.