A method for determination of at least one of the triglyceride molecular species in a biological sample comprising the subjecting sample to lipid extraction to obtain a lipid extract and subjecting the resulting lipid extract to 2D electrospray ionization tandem mass spectrometry with neutral loss scanning of all naturally occurring aliphatic chains and contour analysis of 2D intercept peaks. A filter plug is inserted into an oil passage opening from the crank journal of a crankcase toward the side of the lower portion of the cylinder, and the crank journal is sealed by a plain bearing being abutted against the filter plug. If a symbol display area displaying a symbol included in a displayed designated winning symbol combination is associated with an existing modifier, the gaming system modifies any award associated with that displayed designated winning symbol combination using the existing modifier and associates another different modifier with that symbol display area for a subsequent play of the game. The compression unit optical signal may include either the traffic with an added reflected channel, or a dropped reflected channel. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the kinase peptides, and methods of identifying modulators of the kinase peptides.