A sloped concrete roof and eave method and system is disclosed which can be used in commercial or residential construction. A plurality of paired nucleic acid sequence reads is received, wherein each read is comprised of a first tag and a second tag separated by an insert region. High frequency noise superimposed on the input signal is thus reduced and a difference between the average values of the plurality of sampling values obtained by sampling is output. The hot runner system may be configured and reconfigured to accommodate various drop or nozzle locations.