A wobble signal generating apparatus of an optical-electronic system. A bounding box containing an object is constructed in a 3-D coordinate system. The asynchronous data is collected at an application layer or at an end-user application layer. The correction signal is subtracted from the second intermediate signal in a subtractor to yield the digital output signal YLOGK). It relates in particular to those methods and compositions that provide for single-step detection of target analytes without the need for labor-intensive steps necessary in conventional assays.