The invention provides polynucleotides including biallelic markers derived from genes involved in arachidonic acid metabolism and from genomic regions flanking those genes. The method can include measuring an actual power consumption of the motor necessary to pump water and overcome losses. A filter pod is supported on its periphery by the support ring during brewing. The core assembly is positioned into a second-shot mold assembly, and a second thermoplastic material is injected into the mold so that the second thermoplastic material extends over and adheres to the core portion and the circuit component.