A device including a shallow chamber for analyzing a target analyte in a body fluid using the signal generated by fluorescent detector molecules specific for the target analyte and attenuating the signal emitted by fluorescent detector molecules non-specifically bound to the surfaces of the chamber by a signal attenuating dye; and method thereof. A refractive index of the cladding portion is substantially equal to that of the inner core layer. In combining the molybdenum disulfide with the canola oil it preferred that the high shear mixing occur in the presence of a magnetic field.