Compositions and methods for improved delivery of macromolecules into eukaryotic cells are provided. The presence of the chemical is determined by using ion mobility spectroscopy. The present invention further relates to an improved fighting area for conducting a hand-to-hand combat sport including a raised platform that is subdivided into a plurality of zones including: a first zone situated at the center of the boxing area; and one or more zones surrounding said first zone. A plurality of clips are mounted on the lever arms to receive rods andor cables from the door handles and lock mechanism so as to control the opening and closing of the latch. The method and apparatus can be extended to more than two layers thus forming a multilayer SOI device having a different crystal orientation at each layer.