An apparatus that allows for separating and collecting a fraction of a sample. Preferably, the guidewire manipulation device includes a powered motor that drives a tandem roller assembly. A diameter of a vent hole which is open within the recess portion is set to be larger than a diameter of a vent hole which is open on the profile of the side forming surface. If one or more objects have moved based upon the scene graph comparison, motion information about the objects is determined and the motion information is forwarded to a stitcher module. To provide additional layers of security, at least one invisible IR or UV image, or both, may also be printed on the printable sheet to provide security andor tracking information.