A method for detecting an anomaly in a biological material is disclosed, including the steps of: irradiating the biological material with electromagnetic radiation of at least two different energy levels; measuring the amount of radiation transmitted through the biological material; and determining, for each energy level, a transmission value. There are also provided a microparticlepolyrotaxane-containing coating film and a coated article. Mutant -lactamase inhibitor proteins may be produced, characterized, isolated and expressed in prokaryotic cells and used as antimicrobial agents. A diversity control unit generates a second switching signal by the built-in hardware circuit. The LDMOSFET also includes an n-type diffusion region in the n-type epitaxial layer, the n-type diffusion region electrically connecting the n-type buried layer with the n-type drift region.