The use of a polyolefin film comprising a polypropylene polymer and a polyethylene polymer for the packaging of fresh produce. The pigtail assembly apparatus includes a fiber-feeding module, peeling module, gluing module, insertion module and heating module, all controlled by a control module. A retaining member is removably coupled to the first end and to the closure member such that the retaining member extends through the first end and engages a recess within the closure member. This indicator displays the temperature of the contents of the supply container so that a user can ensure proper dispensing of the reactive components. This method involves altering one or more singletons in the parental AAV capsid to conform the singleton to the amino acid in the corresponding position of the aligned functional AAV capsid sequences. In one embodiment, the invention includes controlled delay queuing logic to hold a packet in a first class for a queuing time of at least a controlled delay value, and scheduling logic to determine whether to delay transmission of a packet in a second class to allow the transmission of the packet in the first class when the queuing time reaches the controlled delay value.