A stateless distributed computer architecture allows state-caching objects, which hold server state information, to be maintained on a client or network rather than on a server. The programmed cells may be pulsed with tumor cell RNA or tumor cell RNA expression products. The present invention also provides for methods for assessing the responsiveness of a patient to an angiogenesis inhibitor by determining the presence of one or more variant alleles of the VEGFR-1 gene. A parallel shaft is positioned parallel to the central shaft.