A hydraulically actuated medical instrument includes an elongated shaft having proximal and distal ends, a hydraulically actuated end effector at the distal end of the shaft and a fluid flow path extending through the shaft to the end effector. The piston has a tip at its distal end and the housing has a distal end sized to receive the tip and also to receive a portion of on of the fasteners. According to the method a warning relating to tire inflation pressure loss emitted by a pressure module of the tire pressure monitoring system with direct measurement is not transmitted to the driver until this tire inflation pressure loss is confirmed by the tire pressure monitoring system with indirect measurement or by all pressure modules that are still working in fail-free manner. A secures mechanism allows the dispenser to be quickly and easily mounted and removed from the top of an aerosol can. Thus, in some cases, latency may be reduced when responding to requests in which anticipatory data has already been provided. The method comprises the step of introducing genomic DNA from a mitochondrially depleted donor cell into a recipient cell from which genomic DNA has been removed.