The present invention related to a method for diagnosing, screening, prognosing and treating bone loss as a result of bone turnover in men and women subjects. A first reflective material is disposed upon a first surface of the substrate. Derivatives made by quaternizing, sulfonating, alkoxylating, sulfating, andor sulfitating the esteramines are also disclosed. The flow-modulation device works in conjunction with the upstream pressure drop devices to aliquot refrigerant from the first plurality of tubes to the second plurality of tubes. In a second embodiment, a seat bottom includes arched leaf springs that supplant the air spring to increase the range of relative movement between the top and bottom plates and to lower the profile of the seat bottom.