A polynucleotide is barcoded using a method whereby an isolated, individual polynucleotide is immobilized on a solid phase and stretched, targets are labeled using target-specific hybridization probes, and an individual label of an unamplified probe at each of the labeled targets is optically detected. The first-type transistors are on first portions of the substrate that have a first type of crystalline orientation and second-type transistors are on second portions of the substrate that have a second type of crystalline orientation. It has been found that such pulses keep metal flowing in a manner that addresses problems, particularly at the beginning of an ingot cast, associated with metal meniscus contracting and pulling away from the mold on the short faces and corners.