A vessel filter comprising a first region and a second region wherein the filter is movable between a collapsed position for delivery to the vessel and an expanded position for placement within the vessel. The allocation plan allocates a first portion of advertisement impressions to satisfy guaranteed demand and a second portion of advertisement impressions to satisfy nonguaranteed demand. A kit for purifying nucleic acid containing a coated matrix and an integrity maintenance provider for preserving the matrix and purifying nucleic acid is also provided. A lubricant filling is possible, which reduces wear and as a result makes a lifetime brake possible. In particular, it was discovered that PTHrP levels in broncho-alveolar lavage are indicative of lung \u201chealth\u201d and \u201cdisease\u201d, and can be used to predict lung disease in patients at risk of chronic lung disease andor to evaluate the efficacy of a ventilation regime.