A method is presented for pruning a peer-to-peer network such that a peer-to-peer search within the peer-to-peer network is initiated beginning with those nodes on which relevant content has previously been found for particular search keywords. Antisense RNA constructs of the present invention employ the use of antisense intron DNA corresponding to distinct intron regions of the gene whose expression is targeted for down-regulation. If the message indicates a change in the number of clients participating in the conference call, the media gateway simultaneously transfers the conference call and plays a prompt to the clients indicating the change. A sensor is attached to the limit structure for generating an electrical signal in response to the sensor being placed in tension.