Embodiments relate to non-disruptive modification of a device mapper stack. Using this graphical user interface, a user may determine, for example, which masterclips are used in a sequence, or which sequences are using those masterclips. The efficacy of the technique for high resolution separation of oligonucleotides is demonstrated for an industrial mixture. A method includes rotating a gate from a first position with a plane of the gate axially aligned with flow to a second position with the disc initially contacting a seat and compensating for the misalignment by pivoting the gate with respect to the lever arm such that the gate squarely contacts the seat. At least one tread element has a sipe that devolves from a constant width sipe having a three dimensional aspect in at least a portion of the radially outer portion of the sipe to a wider width groove at the base of the tread feature.