The present invention provides a method for performing channel estimation and detection in a Multiple-Input-Multiple-Output system, including: generating a demodulation reference signal, DM-RS, pattern for an extended cyclic prefix ; and estimating and detecting channel performance based on the generated DM-RS signal pattern. The securing device has a casing for tightly securing a plug to a socket and fitting various sizes of plugs. An adaptive system which senses and responds to a clogged condition in the grate can be provided by sensing crusher motor current or, in the alternative, sensing grate bar deflection using strain gage transducers. Tiles with front glass panels that are relatively thick may be utilized without unduly blocking light emitted from the display or creating relatively visible seams between adjacent tiles. The flow control valve has an open position and a recirculate position, wherein the first inlet is coupled to the first outlet when the flow control valve is in either the open position or the recirculate position. The invention also provides host cells and methods for producing VGF polypeptides.