A method for detecting protein present on the surface of a sample is disclosed, wherein substances present on a portion of said surface are transferred to a sampling means and wherein, subsequently, these substances are contacted with a reagent capable of forming or changing color upon reaction with protein. The AV stream stored in the local HD includes an audio stream for an additional language. A tracer key is created based on this information. At least one sub-unit probability and at least on large unit probability from the acoustic model are used by a decoder to generate a score for a sequence of hypothesized words. Gain control is used to limit incoming signal magnitude to a specified range of magnitudes.