Methods for use of functionalized fluorescent nanoparticles in the non-destructive, direct and dynamic determination of free volume and glass transition changes and mechanical property changes of polymers and polymer composites are provided. A portion of the release member is temporarily secured to the strap at a retention location spaced from the free end. The resulting liquid crystal systems are readily applicable onto a substrate to obtain optically isotropic or anisotropic, at least partially crystalline films applicable in various fields. The frame assembly is fixedly attached to front and back surface protection members, which increase the resistance to wind-induced forces and the impact of objects. A direction and size of the graphical pattern are unknown.