Reducing the number of inquiries required to determine access rights accorded to documents, in particular documents stored at a Web site, is disclosed. The method further includes modulating the IVPA data to determine a first tissue border and displaying a border map representing the first tissue border. A second storage is maintained at at least one of the primary and secondary sites. The lactone based compound comprises substituents selected from the group consisting of alkyl groups, alkenyl groups, alkynyl groups, aryl groups, and combinations thereof.