An apparatus for placing marks on a resurfaced roadway. The puncture needle is adapted to be stuck into the skin along the guide member. When the female connector housing is coupled to the male connector housing, the tab protection member is forcibly moved from the temporary engagement position to the final engagement position by the female connector housing and the tabs penetrate the tab through-holes, thereby coupling the tabs to terminals in the female connector housing. Next, the card is inserted into the housing space. Also disclosed are methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention. This can result in rotation of the set of transfer rollers and application of a discharge force to items set upon them.