A multi-layer sheet material comprises a film, and adhesive layer and an optional release liner. A polarizing beam splitter combines S-polarized-light from the first light source and P-polarized light from the second light source. The probes or leads of electrical testing equipment are inserted into apertures in the apparatus. The voltage offset is a predetermined function of whether none or one or both of its neighbors are in a mode that creates perturbation, such as in a program inhibit mode. Sequestrin protein and sequence can be used as a diagnostic, prognostic, and therapeutic tool. Also described is a loss detection technique using sequence numbers.