A refrigerator freezer having an ice maker positioned on a refrigerator compartment or freezer compartment door. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence. Secondly, a band of sealant adjacent the top edge of an underlying shingle seals against an overlying shingle to prevent penetration by wind driven rain to the roof deck. Further, a resulting number of non-zero bits in the modified set of coefficients is quantified.