The present invention provides methods for detecting disease by analysis of a patient sample to determine the integrity of nucleic acids in the sample. The message processing system allows for the receipt, transmission and management of messages. An ordinary garden hose is attached to a hose bib outside the screened enclosure and to the hose bib to garden hose adapter providing the water supply. Both the first and second electrical potentials are transmitted to the jaw members through the first shaft. The apparatus includes a handle, an elongated body connected to the handle, a pair of jaws attached to the elongated body, a rod persuader for advancing the rod toward the anchoring device, and a locking shaft for use in locking the locking cap against the anchoring device to secure the spinal rod relative to the anchoring device. The leader node causes the originating node to transmit the telephone call to the receiving node using the IP network.