\u201cAn air brake hose coupling member includes a locking device having a housing with a first aperture for attachment to the air brake hose coupling member and a second aperture for attachment to a hose support member which is adjusted to provide a predetermined clearance between the air brake hose coupling and a rail track. The apparatus includes a housing assembly that defines an enclosure, a control system mounted in the housing assembly, and an operator interface mounted on the housing assembly and connected to the control system to allow an operator to control the measuring apparatus. For example, functional molecules can be linked to a peptide to provide a peptide conjugate having differing and useful properties. The ARFN may then cause that operation to be performed within an environment.