The present invention provides methods for assembling DNA molecules in a predetermined order to produce a DNA construct useful in Agrobacterium mediated transformation in plants. This device comprises a first protection means for rejecting electrostatic discharges on the first path, a first rejection means for rejecting radio signals at least in a chosen frequency band on the first path, and a first AC-coupling means for interfacing voltage levels between the first audio signal source and the audio signal processing means. The side-port sheath may include a suspension ribbon used to deploy, or that aids in the deployment of, the embedded gliding catheter. A heat source extends into the column of modified material and is operable to provide heat to the hydrate formation so as to release methane gas from the hydrate formation. A single auction minimum price may be determined for an auction using the minimum prices assigned to satisfied serving constraints of ads participating in the auction. The relationship Rp=\u0394V\u0394I is calculated, where Rp is the polarization resistance of the working electrodes and is a measure of generalized corrosion rate.